EpiAirway是现成的, 三维粘液纤毛组织模型组成正常, human-derived tracheal/bronchial epithelial cells also available as a co-culture system with normal human stromal fibroblasts (EpiAirwayFT). 气液界面培养(ALI), epi气道概述了屏障的体内表型, 黏膜纤毛的反应, infection, 毒性反应和疾病. 有超过100个技术参考, EpiAirway is amenable to acute or long-term chronic studies across a wide range of highly predictive in vitro applications.
EpiAirway exhibits human relevant tissue structure and cellular morphology with high uniformity and reproducibility. 其三维结构由有组织的角蛋白5+基底细胞组成, 产生粘液的杯状细胞, 功能紧密的连接和跳动的纤毛. EpiAirwayFT incorporates human fibroblasts in an extracellular stromal matrix ideal for inflammation and fibrosis research.
The EpiAirway 3D human tissue model is routinely utilized for a range of applications including safety & 风险评估，抗病毒研究，炎症 & 纤维化和药物输送. Simple protocols and the evaluation of early cellular endpoints allow scientists to acquire data in days, not weeks or months.
Virus Infection
使用EpiAirway研究呼吸道病毒附着, replication, 先天免疫反应和抗病毒药物的开发.
Drug Delivery
Utilize existing transdermal permeation equipment or 365体育网投’s simple single insert permeation devices to assess API permeation and flux.
吸入毒物学
Use EpiAirway (available in several formats for physiological exposure systems) to determine relative safety with the EpiAirway IC-75 assay.
炎症和纤维化
Employ EpiAirway’s diverse donor inventory of healthy and diseased donors (Asthma, COPD, Smoker, Goblet Cell Hyperplasia) to elucidate molecular pathways and assess therapeutic efficacy.
Tissue
Kit: 标准的EpiAirway试剂盒(AIR-100)由24个组织组成. (组织“包”包含组织, 少量培养基, and plasticware; contact 365体育网投 for specific kit contents)
Formats:
- 9mm & 22mm individual inserts – tissue culture substrate is chemically modified with a pore size of 0.4 μm
- 24mm, 12mm & 6.5mm Transwell插孔，孔径为0.4 μm
- 96-well HTS plates – tissue culture substrate is chemically modified with a pore size of 0.4 μm
Culture: 气液界面
Histology: 3-4 cell layers – pseudostratified, mucociliary morphology and tissue structure
Lot numbers: Tissue lots produced each week are assigned a specific lot number. A letter of the alphabet is appended to the end of the lot number to differentiate between individual kits within a given lot of tissues. 所有的组织包在细胞方面都是相同的, medium, handling, culture conditions, etc.
Shipment: 4°C，培养基中添加琼脂糖凝胶
Shipment day: Every Monday
Delivery: 周二上午，通过联邦快递优先服务(美国). 美国以外:周二-周四，视地点而定
Shelf life: Including time in transit, tissues may be stored at 4°C for up to 3 days prior to use. However, extended storage periods are not recommended unless necessary. In addition, the best reproducibility will be obtained if tissues are used consistently on the same day, e.g. Tuesday afternoon or following overnight storage at 4°C (Wednesday morning)
长度的实验: Tissue cultures can be continued for THREE (3) MONTHS or more with good retention of normal morphology. 面巾纸必须每隔一天用5.0ml维护介质(AIR-100-MM). Cell culture inserts are placed atop washers (EPI-WSHR) or culture stands (MEL-STND) in 6-well plates to allow us of 5.0 ml. See 技术参考# 631.
Cells
Type: 正常人气管/支气管上皮细胞(NHBE);
Genetic make-up: Single donor
Derived from: 健康，不吸烟(可根据要求提供替代供体)
Alternatives: 来自哮喘、COPD和吸烟者的NHBE捐赠者
Screened for: 艾滋病毒，乙型肝炎，丙型肝炎，支原体
Medium
Base medium: 杜尔贝科改良Eagle中型(DMEM)
生长因子/激素: 表皮生长因子和其他专有因子
Serum: None
Antibiotics: 庆大霉素5µg/ml(庆大霉素正常水平的10%)
Anti-fungal agent: Amphotericin B 0.25 µg/ml
pH Indicator: Phenol red
Other additives: Proprietary
Alternatives: Phenol red-free, antibiotic-free, anti-fungal-free medium and tissues are available. 代理至少在发货前3天被移除.
Assay medium: AIR-100-ASY
Maintenance medium: AIR-100-MM
质量控制和无菌
Visual inspection: All tissues are visually inspected and if physical imperfections are noted, 组织被拒绝装运
End-use testing: Transepithelial electrical resistance (TEER) of each EpiAirway lot is measured using an EVOM2 epithelial Volthommeter and Endohm electrode chamber (World Precision Instruments). 最小TEER为300欧姆•cm2 QC放行需要什么.
Sterility: All media used throughout the production process is checked for sterility. Maintenance medium is incubated without antibiotics for 1 week and checked for sterility. The agarose gel from the 24-well plate used for shipping is also incubated for 1 week and checked for any sign of contamination
筛查病原体: All cells are screened and are negative for HIV, hepatitis B and hepatitis C using PCR. However, no known test method can offer complete assurance that the cells are pathogen free. Thus, these products and all human derived products should be handled at BSL-2 levels (biosafety level 2) or higher as recommended in the CDC-NIH manual, “微生物和生物医学实验室的生物安全,” 1998. For further assistance, please contact your site Safety Officer or 365体育网投 technical service
地段失败的通知: 如果一个组织批次没有通过我们的质量控制或无菌测试, the customer will be notified and the tissues will be replaced without charge when appropriate. 因为我们的QC和无菌测试是在出货后进行的, notification will be made as soon as possible (Under normal circumstances, TEER故障将在周三下午5点前通知.m.; sterility failures will be notified within 8 days of shipment)
