Engineered to enable in vitro skin research in which fibroblast-keratinocyte cell interactions are important, 365体育网投公司的表皮mft系统由正常的, 人表皮角化细胞(NHEK)正常, human dermal fibroblasts (NHFB) cultured to form a multilayered model of the human dermis and epidermis. Cultured at the air-liquid interface in easy-to-handle tissue culture inserts, EpiDermFT attains levels of differentiation at the cutting edge of tissue engineering technology.
表皮mft由表达基底细胞的有组织的角蛋白5组成, Involucrin和角蛋白10表达棘状和颗粒层, 角化表皮层与体内发现的相似. The dermal compartment is composed of a collagen matrix containing viable normal human dermal fibroblasts (NHDF). The epidermal and dermal layers are mitotically and metabolically active and exhibit in vivo-like morphological and growth characteristics which are uniform and highly reproducible.
A 成熟的基底膜 是否存在于真皮/表皮交界处. Hemidesomosomes(›), lamina lucida, lamina densa(→) and anchoring fibril structures(») are evident by transmission electron microscopy.
EpiDermFT tissues are used across a wide range of applications including scientific efficacy and claims substantiation. 由于皮肤表面干燥, EpiDermFT can be used to evaluate biological responses to topical applications of formulations or ingredients. Simple protocols and the evaluation of early molecular endpoints allow research to acquire data in days, not weeks or months.
Anti-Aging
Examine changes in extracellular matrix gene and protein expression and histological structure to determine the anti-aging efficacy of cosmetic ingredients or final formulation. 表皮mft可从各种成人捐献者获得.
Wound Healing
使用表皮mft评估治疗与伤口愈合检测试剂盒. Wound healing assay kits include 24 pre-wounded tissues and are available with epidermal only wounds or full-thickness wounds of the epidermis and dermis.
Skin Hydration
Use EpiDermFT to assess the efficacy of topically applied moisturizers by measuring the electrical impedance of the epidermis. Amenable to creams, gel, 乳液或其他配方, 皮肤水合试验允许在24小时内进行数据分析.
UV Protection
分析环嘧啶二聚体的形成, inflammatory mediator release and oxidative endpoints to evaluate the UV protective efficacy of compounds or formulations.
Browse our reference library 来看看我们的表皮mft组织是如何在这些研究领域中使用的.
Tissue
Kit: 一个标准的表皮mft试剂盒(EFT-400)包含24个组织. (组织“包”包含组织, 少量培养基, and plasticware; contact 365体育网投 for specific kit contents)
Substrate: 使用Costar Snapwell™单孔组织培养板插入. Pore size = 0.4 μm, Diameter = 1.2 cm. Surface area = 1.0 cm2
Culture: Air-liquid interface
Histology: 8-12 cell layers plus stratum corneum (basal, spinous, and granular layers)
Lot numbers: 每周生产的组织批号都有一个特定的批号. A letter of the alphabet is appended to the end of the lot number to differentiate between individual kits within a given lot of tissues. 所有的组织包在细胞方面都是相同的, medium, handling, culture conditions, etc.
Shipment: 4°C，培养基补充，琼脂糖凝胶6孔板
Shipment day: Every Monday. 如有特殊要求，也可于周四装运
Delivery: 周二上午，通过联邦快递优先服务(美国). 美国以外:周二-周四，视地点而定
Shelf life: Including time in transit, tissues may be stored at 4°C for up to 6 days prior to use. 但是，除非必要，不建议延长储存时间. In addition, the best reproducibility will be obtained if tissues are used consistently on the same day, e.g. Tuesday afternoon or following overnight storage at 4°C (Wednesday morning)
Length of experiments: Cultures can be continued for up to 2 weeks with good retention of normal epidermal morphology. 培养物必须每隔一天用5.0 ml of EFT-400 medium
Cells
Type: Normal human epidermal keratinocytes (NHEK); Normal human dermal fibroblasts (NHDF)
Genetic make-up: Single donor
Derived from: Neonatal-foreskin tissue (NHEK); Neonatal skin (NHDF)
Alternatives: NHEK from adult breast skin; NHDF from adult skin
Screened for: 艾滋病毒，乙型肝炎，丙型肝炎，支原体
Medium
Base medium: 杜尔贝科改良Eagle中型(DMEM)
生长因子/激素: 表皮生长因子, insulin, hydrocortisone and other proprietary stimulators of epidermal differentiation
Serum: None
Antibiotics: 庆大霉素5µg/ml(庆大霉素正常水平的10%)
Anti-fungal agent: Amphotericin B 0.25 µg/ml
pH Indicator: Phenol red
Other additives: Lipid precursors used to enhance epidermal barrier formation (proprietary)
Alternatives: 苯酚red-free (eft - 400编码脉冲), 不需抗生素(eft - 400沛富), anti-fungal-free (eft - 400等于off), 或无氢化可的松培养基和组织(EFT-400-HCF)是可用的. 代理至少在发货前3天被移除
分析/维护媒介: EFT-400-ASY is utilized for assays; EFT-400-MM is used for term maintenance of the EFT-400 tissues
质量控制和无菌
Visual inspection: All tissues are visually inspected and if physical imperfections are noted, 组织被拒绝装运
End-use testing: 组织暴露于1% Triton X-100 8、12、16和24小时. The time of exposure required to reduce the tissue viability (ET-50) using the MTT viability assay is determined (See 365体育网投 EpiDermFT use protocol) for each lot of tissue. ET-50一般在6的范围内.5-9.5 hours. 客户实验室的ET-50可能与365体育网投的结果略有不同
Sterility: All media used throughout the production process is checked for sterility. Maintenance medium is incubated with and without antibiotics for 1 week and checked for sterility. The agarose gel from the 24-well plate used for shipping is also incubated for 1 week and checked for any sign of contamination
筛查病原体: All cells are screened and are negative for HIV, hepatitis B and hepatitis C using PCR. However, no known test method can offer complete assurance that the cells are pathogen free. Thus, these products and all human derived products should be handled at BSL-2 levels (biosafety level 2) or higher as recommended in the CDC-NIH manual, “微生物和生物医学实验室的生物安全,” 1998. For further assistance, please contact your site Safety Officer or 365体育网投 technical service
地段失败的通知: 如果一个组织批次没有通过我们的质量控制或无菌测试, the customer will be notified and the tissues will be replaced without charge when appropriate. 因为我们的QC和无菌测试是在出货后进行的, notification will be made as soon as possible (Under normal circumstances, ET-50失败将在周三下午5点前通知.m.; sterility failures will be notified within 8 days of shipment)
